Generation of moderate amounts of polyclonal antibodies in mice.
نویسندگان
چکیده
D. Stuber and K. Henco. 1994. 6xHis-NiNTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol. Biol. 31:371-387. 3.Galibert, F., E. Mandart, F. Fitoussi, P. Tiollais and P. Charnay. 1979. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281:646-650. 4.Gentz, R., C.-H. Chen and C.A. Rosen. 1989. Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis. Proc. Natl. Acad. Sci. USA 86:821-824. 5.Smith, D.B. 1993. Purification of glutathioneS-transferase fusion proteins. Methods Mol. Cell Biol. 4:220-229. 6.Smith, D.B. and K.S. Johnson. 1988. Singlestep purification of polypeptides expressed in Escherichia coli as fusions with glutathione Stransferase. Gene 67:31-40.
منابع مشابه
Tissue Distribution of 125I-human Nonspecific Polyclonal IgG in Normal and Induced Inflammation Mice
Many different radiolabeled antibodies have been used for radioimmunotherapy and radioimmunoscintigraphy of human diseases in animal experiments. In order to study the in vivo tissue distribution of antibody, we labeled human nonspecific polyclonal IgG with Na125I using chloramine-T method. An animal model was developed by injecting turpentine in the posterior left thigh of Balb/c mice. Tissue ...
متن کاملTissue Distribution of 125I-human Nonspecific Polyclonal IgG in Normal and Induced Inflammation Mice
Many different radiolabeled antibodies have been used for radioimmunotherapy and radioimmunoscintigraphy of human diseases in animal experiments. In order to study the in vivo tissue distribution of antibody, we labeled human nonspecific polyclonal IgG with Na125I using chloramine-T method. An animal model was developed by injecting turpentine in the posterior left thigh of Balb/c mice. Tissue ...
متن کاملMonoclonal Antibodies
SUMMARY Monoclonal Antibodies are identical antibodies and Can be generated in ultimated amounts by countinuos cultures of single antibody- secreting cells. These cell lines are produced by cell fusion of normal lymphocytes to cells of a myeloma tumor cell line, which confers on the antibody producing hybrid cell, immortality and the ability grow as atumor in animals. monoclonal antibodies c...
متن کاملMonoclonal and Polyclonal Antibodies Specific to Human Fibromodulin
Background: The unique expression of fibromodulin (FMOD) in patients with chronic lymphocytic leukemia (CLL) has been previously reported. Detecting FMOD in CLL patients using specific anti-FMOD mAbs might provide a promising method in detection, monitoring, and prognosis of CLL. Objectives: In this study, we aimed for producing specific antibodies agains...
متن کاملPreparation and Purification of Polyclonal Antibodies against Mycobacterium Avium Paratuberculosis Antigens in Rabbit
Background and Objective: Johne’s disease is the chronic granulomatous enteritis of ruminants, and a major health hazard worldwide. In recent years, researchers have focused on mycobacterium avium subsp. paratuberculosis (MAP) antigens in diagnostic tests. Identification of antibodies against MAP antigens is, therefore, effective for the diagnosis or preparation of vaccine. The aim of this stud...
متن کاملPurification of human anti-erythropoietin polyclonal antibodies by precipitation and chromatography as an optimized method with potential application in vaccine studies
Introduction: Polyclonal antibodies are required to be affinity purified. Improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. Two common techniques for purifying proteins is salt precipitation and chromatography purification. Our work fo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- BioTechniques
دوره 23 4 شماره
صفحات -
تاریخ انتشار 1997